wrl68 human embryonic liver cells  (ATCC)

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The effects of vincristine on WRL68 cells and cell viability were assessed using the CCK-8 method. Cells were treated with different concentrations of vincristine (0.00, 0.01, 0.10, 1.00, 5.00, and 10.00 μg/mL) for 48 h at 37 °C. Data are presented as the means ± SEM determined from five independent experiments. **** P < 0.0001compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: CCK-8 Assay, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The averaged absorption spectra of WRL68 cells that were untreated (control group, black) and treated with 0.120 μg/mL vincristine (treated group, red).

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The vector-normalized averaged second-derivative spectra of the WRL68 cells that were untreated (control, black) and treated with vincristine (red).

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from the WRL68 cells in the lipid region (3000–2800 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.925, R 2 Y = 0.704, Q 2 = 0.651). The x - and y -axes indicate the first component t1 and the first orthogonal component to1. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean ν as (CH 2 )/ν as (CH 3 ) and ν s (CH 2 )/ν s (CH 3 ) peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated cells, **** P < 0.0001compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from WRL68 cells in the protein region (1800–1480 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.961, R 2 Y = 0.617, Q 2 = 0. 511). The x - and y -axes indicate the first component t1 and the first orthogonal component to1, respectively. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean protein amide I/amide II (1670–1646/1563–1531) and α-helix/β-sheet (1670–1646/1644–1621) absorption band peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated cells. **** P < 0.0001 and *** P < 0.001 compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from the WRL68 cells in the fingerprint region (1480–900 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.597, R 2 Y = 0.873, Q 2 = 0. 754). The x - and y -axes indicate the first component t1 and the first orthogonal component to1, respectively. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean nucleic acid ν as (PO 2 – )/lipid ester ν(C=O) (1269–1201/1754–1723), nucleic acid ν as (PO 2 – )/protein amide II (1269–1201/1563–1531), nucleic acid ν s (PO 2 – )/lipid ester ν(C=O) (1105–1070/1754–1723), nucleic acid ν s (PO 2 – )/protein amide II (1105–1070/1563–1531), and nucleic acid ν s (dianionic phosphate monoester)/lipid ester ν(C=O) (984–948/1754–1723) peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated groups. **** P < 0.0001 compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: Assignments of the Most Relevant IR Absorption Bands of WRL68 Cells, Together with the Related Vibrational mode 28 − 30

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: